How to Choose a Sequencer
NGS System Buyer’s Guide
Use this guide to help you evaluate important factors and choose the right next-generation sequencing instrument for your lab.
Sequencing Platform Comparison Tool
This interactive tool will help you compare sequencers and find the one that’s right for your lab or application.
Benchtop Sequencer Comparison Table
Compare the iSeq 100 System with other Illumina benchtop sequencers.
Next-Generation Sequencing Education
Introduction to NGS
This detailed overview describes the basics of Illumina sequencing technology, key methods, and more.
Introduction to Sequencing by Synthesis
See Illumina sequencing by synthesis (SBS) technology in action and learn how it works.
NGS vs. Sanger Sequencing
Learn the key differences between NGS and Sanger sequencing and determine when NGS can be a more effective option.
NGS vs. qPCR
Compare the benefits of NGS technology to qPCR and learn which one to choose.
NGS Library Preparation
Find kits, methods, and automation solutions to help you prepare DNA or RNA libraries before sequencing.
Sequencing Data Analysis
Our software simplifies sequencing data analysis, so you can focus on your research instead of setting up analysis workflows.
Benefits of Targeted Resequencing
Learn how targeted NGS can help you gain greater insight in your studies and save time compared to traditional methods.
One-Channel SBS
See how sequencing on a semiconductor chip reduces instrument cost and simplifies NGS on the compact iSeq 100 System.
Beginner's Guide to Next-Generation Sequencing
Considering bringing next-generation sequencing to your lab, but unsure where to start? These resources cover key topics in NGS and are designed to help you plan your first experiment.
Learn More
Financing Your Research
Instrument Buying Options
Explore different buying options—including leasing, bundles, trade-in offers, and more—to find one that fits your needs.
Grant Writing Assistance
This document offers specification information and other resources to help you justify funding for the iSeq 100 System.
Illumina Financial Solutions
Acquire the advanced technology you need now through fast, easy, and affordable financing and leasing programs.
Cost of NGS
Next-generation sequencing costs have decreased dramatically. Find resources to help you plan your experimental budget.
Support and Training
iSeq 100 System Manuals and Documentation
Find supporting documentation for the iSeq 100 System.
Sequencing Online Training
Online Illumina sequencing courses are free, interactive, and available any time.
Sequencing Coverage
Sequencing coverage describes the average number of NGS reads that align to known reference bases. Coverage requirements vary by application.
Quality Scores
Additional information about sequencing quality scores, how they are calculated, and the relationship between quality score and base call accuracy.
NGS Experimental Design
Find resources to help you design, run, and evaluate your sequencing project—everything you need to plan your experiment.
NGS Tutorials
These tutorials are designed to help you understand key concepts in next-generation sequencing.
Selection Tools
Library Prep Kit Selector
Determine the best sequencing library preparation kit for your needs based on your project type, starting material, and method of interest.
Gene Panel Finder
Identify sequencing panels that target your genes of interest.
Product Selection and Experiment Planning Tools
Find interactive tools designed to help you choose the right products for your needs and plan your experiments.
iSeq 100 Educational Resources
System Literature
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Microbial Whole-Genome Sequencing App NoteApplication note | PDF | 5 MB
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Illumina CMOS Chip and One-Channel SBS Chemistrytechnology_spotlights | PDF | < 1 MB
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Grant Assistance DocumentProduct information sheet | PDF | < 1 MB
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iSeq 100 System BrochureBrochure | PDF | < 1 MB
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AmpliSeq for Illumina Oncology Panels on the iSeq 100 SystemApplication note | PDF | < 1 MB
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16S Metagenomics Sequencing on the iSeq 100 SystemApplication note | PDF | 4 MB
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Sequencing Library QC on the iSeq SystemApplication note | PDF | < 1 MB
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Illumina Proactive Service connectivity guide - iSeq 100Brochure | PDF | < 1 MB
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Pan-Viral Sequencing on Illumina Benchtop Systems Application NoteApplication note | HTML |
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QC and rebalancing of single-cell gene expression libraries using the iSeq™ 100 SystemApplication note | PDF | 2 MB
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Brochure | PDF | 3 versions
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Accurate surveillance of healthcare-acquired infectionsTechnical note | PDF | < 1 MB
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Technical note | PDF | 11 versions
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Technical note | PDF | 9 versions
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Illumina Proactive on iSeq 100 SystemBrochure | PDF | < 1 MB
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Microbial whole-genome sequencing with the iSeq 100 SystemApplication note | PDF | < 1 MB
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Illumina DNA PrepData sheet | PDF | < 1 MB
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iSeq 100 System specification sheetData sheet | PDF | < 1 MB