Sequencing Library Preparation Methods

Sequencing Method Explorer

Use this interactive tool to explore experimental NGS library prep methods compiled from the scientific literature. The tool includes a subset of methods from the review articles and posters below. New methods will be added as they become available.

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Sequencing Method Posters

View illustrated schematics of DNA, RNA, and single-cell library preparation techniques that are compatible with SBS technology. Also see brief diagrams of Illumina library preparation kit protocols.

For All You Seq—DNA

This poster includes NGS methods for analyzing:

DNA variants
Low levels of DNA
Epigenetics
DNA–protein interactions
Protein–protein interactions

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For All You Seq—RNA

This poster includes a comprehensive set of NGS methods for analyzing:

RNA transcription
RNA–protein interactions
Low levels of RNA
RNA modifications
RNA structure

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For All You Seq—Single-Cell

This poster includes a comprehensive set of NGS-based single-cell analysis methods for:

Low-level RNA detection
Low-level DNA detection
Integrated DNA and RNA analysis

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Featured Method

scTrio-Seq

scTrio-Seq can analyze genomic CNVs, the DNA methylome, and the transcriptome of an individual mammalian cell simultaneousl. This approach is an extension of previous methods, such as scMT-seq.

Pros

Accurately analyzes the mechanism by which the transcriptome, genome, and DNA methylome regulate each other
CNVs can be identified reliably using scRRBS data

Cons

Lower transcriptome coverage than scMT-seq¹
Results in 3'-biased transcriptome

View References and Review

References

Most Recent Reviews

SBS Technology

Learn more about the advantages of sequencing by synthesis, how it works, and the many ways you can leverage sequencing technology to help answer your research questions.

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Additional Resources

Par-CLIP

Photoactivatable ribonucleoside–enhanced crosslinking and immunoprecipitation (PAR-CLIP) maps RNA-binding proteins (RBPs). This approach is similar to HITS-CLIP and CLIP-Seq, but uses much more efficient crosslinking to stabilize the protein-RNA complexes. The requirement to introduce a photoactivatable ribonucleoside limits this approach to cell culture and in vitro systems. In this method, 4-thiouridine (4-SU) and 6-thioguanosine (6-SG) are incorporated into transcripts of cultured cells. UV irradiation crosslinks 4-SU/6-SG–labeled transcripts to interacting RBPs. The targeted complexes are immunoprecipitated and digested with RNase T1, followed by Proteinase K, before RNA extraction. The RNA is reverse-transcribed to cDNA and sequenced. Deep sequencing of cDNA accurately maps RBPs interacting with labeled transcripts.

View Current Method

Sequencing Library Preparation Methods

NGS library prep methods

An extensive collection of published techniques for preparing sequencing libraries

Sequencing Method Explorer

Sequencing Method Posters

For All You Seq—DNA

For All You Seq—RNA

For All You Seq—Single-Cell

Featured Method

scTrio-Seq

Pros

Cons

References

Most Recent Reviews

SBS Technology

Interested in receiving newsletters, case studies, and information on genomic analysis techniques?

Additional Resources

Method of the Week

Par-CLIP

For every lab, everywhere

For every lab, everywhere

For every lab, everywhere

For every lab, everywhere

For every lab, everywhere

For every lab, everywhere

For every lab, everywhere

For every lab, everywhere

For every lab, everywhere

For every lab, everywhere

Take a Tour with Professor Michael Bunce

Take a Tour with Professor Michael Bunce

Take a Tour with Professor Michael Bunce

Take a Tour with Professor Michael Bunce

Take a Tour with Professor Michael Bunce

The Weizmann Institute Uses NovaSeq to Drive Single Cell Research

The Weizmann Institute Uses NovaSeq to Drive Single Cell Research

The Weizmann Institute Uses NovaSeq to Drive Single Cell Research

The Weizmann Institute Uses NovaSeq to Drive Single Cell Research

The Weizmann Institute Uses NovaSeq to Drive Single Cell Research

For every lab, everywhere

For every lab, everywhere

For every lab, everywhere

For every lab, everywhere

For every lab, everywhere

For every lab, everywhere

For every lab, everywhere

For every lab, everywhere

For every lab, everywhere

For every lab, everywhere

For every lab, everywhere

For every lab, everywhere

For every lab, everywhere

For every lab, everywhere

For every lab, everywhere

For every lab, everywhere

For every lab, everywhere

Sequencing Library Preparation Methods

NGS library prep methods

An extensive collection of published techniques for preparing sequencing libraries

Sequencing Method Explorer

Sequencing Method Posters

For All You Seq—DNA

For All You Seq—RNA

For All You Seq—Single-Cell

Compare a Broad Range of Methods

Featured Method

scTrio-Seq

Pros

Cons

References

Most Recent Reviews

SBS Technology

Interested in receiving newsletters, case studies, and information on genomic analysis techniques?

Additional Resources

Method of the Week

Par-CLIP