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TruSight One Sequencing Panel Kits Contents & Storage

TruSight One Sequencing Panel Contents (9 Samples)

Box 1 Rapid Capture Reagents
Quantity Reagent Description Storage
1 SPB Sample Purification Beads 2°C to 8°C
2 SMB Streptavidin Magnetic Beads 2°C to 8°C
1 ET2 Elute Target Buffer 2 2°C to 8°C
1 ST Stop Tagment Buffer 2°C to 8°C
Box 2 Rapid Capture Reagents, Store at -25°C to -15°C
Quantity Reagent Description
1 TDE1 Tagment DNA Enzyme
1 EE1 Enrichment Elution Buffer
1 TD Tagment DNA Buffer
1 RSB Resuspension Buffer
1 NLM Nextera Library Amplification Mix
1 EHB Enrichment Hybridization Buffer
1 EWS Enrichment Wash Solution
1 HP3 2N NaOH
1 PPC PCR Primer Cocktail
2 NEM Nextera Enrichment Amplification Mix
Box 3 Indexes, Store at -25°C to -15°C
Quantity Reagent Description
2 i5 Index Primers E503 to E504
3 i7 Index Primers N701, N705, and N709
Box 4 Oligos, Store at -25°C to -15°C
Quantity Reagent Description
1 TOO TruSight One Content Set
MiSeq Reagent Kit v3 Box 1, Store at -25°C to -15°C
Quantity Component
3 (HT1) Hybridization Buffer
3 Reagent Cartridge
MiSeq Reagent Kit v3,Box 2,Store at 2°C to 8°C
Quantity Component
3 MiSeq Flow Cell
3 Reagent Cartridge

TruSight One Sequencing Panel or TruSight One Expanded Sequencing Panel Contents (36 Samples)

Box 1 Rapid Capture Reagents
Quantity Reagent Description Storage
2 SPB Sample Purification Beads 2°C to 8°C
2 SMB Streptavidin Magnetic Beads 2°C to 8°C
1 ET2 Elute Target Buffer 2 2°C to 8°C
1 ST Stop Tagment Buffer 2°C to 8°C
Box 2 Rapid Capture Reagents, Store at -25°C to -15°C
Quantity Reagent Description
2 TDE1 Tagment DNA Enzyme
1 EE1 Enrichment Elution Buffer
1 TD Tagment DNA Buffer
1 RSB Resuspension Buffer
2 NLM Nextera Library Amplification Mix
1 EHB Enrichment Hybridization Buffer
1 EWS Enrichment Wash Solution
1 HP3 2N NaOH
1 PPC PCR Primer Cocktail
2 NEM Nextera Enrichment Amplification Mix
Box 3 Indexes, Store at -25°C to -15°C
Quantity Reagent Description
4 i5 Index Primers E503 to E504
12 i7 Index Primers N701, N705, and N709
Box 4 Oligos, Store at -25°C to -15°C
Quantity Reagent Description
1 TOO TruSight One Content Set
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